A mutant of Prevotella ruminicola B14 deficient in β-1,4-endoglucanase and mannanase activities
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منابع مشابه
Ruminal Bacterium Prevotella ruminicola 23t
When PrevoteUa ruminicola 23 was grown in a defined medium containing a vitamin mixture, significant amounts of propionate were formed. Succinate and acetate were the major fermentation acids produced when vitamins were omitted, and further experiments demonstrated that propionate formation was dependent on vitamin B12. When the organism was grown in continuous culture at dilution rates of less...
متن کاملStructural organization of pRAM4, a cryptic plasmid from Prevotella ruminicola.
A total of 530 strains of rumen bacteria were screened for the presence of plasmid DNA. The percentage of plasmid-bearing strains was found to be the highest among the Bacteroides/ Prevotella group (9.9%), while it was less than 1% in the Butyrivibrio (0.2%) and Clostridium (0.6%) genera. A small cryptic plasmid pRAM4 from Prevotella ruminicola T31 was subcloned in Escherichia coli and complete...
متن کاملInteractions between Fibrobacter succinogenes, Prevotella ruminicola, and Ruminococcus flavefaciens in the digestion of cellulose from forages.
The synergistic and inhibitory interactions observed between Fibrobacter succinogenes A3c, Prevotella ruminicola H2b, and Ruminococcus flavefaciens B34b in the digestion of forage cellulose were studied in detail. Orchardgrass and alfalfa hays, both at two maturity stages, were used as substrates. Sequential inoculation procedures were developed whereby a second inoculation was made after the i...
متن کاملIsolation and Characterization of a New Peroxisome Deficient CHO Mutant Cell Belonging to Complementation Group 12
We searched for novel Chinese hamster ovary (CHO) cell mutants defective in peroxisome biogenesis by an improved method using peroxisome targeting sequence (PTS) of Pex3p (amino acid residues 1–40)-fused enhanced green fluorescent protein (EGFP). From mutagenized TKaEG3(1–40) cells, the wild-type CHO-K1 stably expressing rat Pex2p and of rat Pex3p(1–40)-EGFP, numerous cell colonies resistant to...
متن کاملPurification, characterization, and expression of multiple glutamine synthetases from Prevotella ruminicola 23.
The Prevotella ruminicola 23 genome encodes three different glutamine synthetase (GS) enzymes: glutamine synthetase I (GSI) (ORF02151), GSIII-1 (ORF01459), and GSIII-2 (ORF02034). GSI, GSIII-1, and GSIII-2 have each been heterologously expressed in and purified from Escherichia coli. The subunit molecular mass of GSI was 56 kDa, while GSIII-1 and GSIII-2 were both 83 kDa. Optimal conditions for...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2006
ISSN: 0378-1097
DOI: 10.1111/j.1574-6968.1997.tb12617.x